Mass spectrometry based proteomics

By Prof. Sanjeeva Srivastava | IIT Bombay

Learners enrolled: 837 | Exam registration: 208

ABOUT THE COURSE

Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio. MALDI and ESI- coupled with mass analyzers are commonly used mass spectrometer configuration in proteomics. The human proteome draft was decoded by using high-resolution liquid chromatography coupled with mass spectrometry.

In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.

INTENDED AUDIENCE

Core course
All UG and PG students can attend this course.
It would be applied to B.Sc., M.Sc. and Ph.D

PRE-REQUISITES

B.Sc. Or M.Sc.
The target audiences of this course are required to have a basic biology and biochemistry background.

Summary

Course Status :	Ongoing
Course Type :	Core
Language for course content :	English
Duration :	4 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	1
Level :	Undergraduate/Postgraduate
Start Date :	16 Feb 2026
End Date :	13 Mar 2026
Enrollment Ends :	16 Feb 2026
Exam Registration Ends :	27 Feb 2026
Exam Date :	25 Apr 2026 IST
NCrF Level	4.5 — 8.0

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1: Proteomics Introduction and Sample Preparation

Lecture 1: Introduction to proteomics

Lecture 2: Why proteomics and sample preparation

Lecture 3: Protein extraction

Lecture 4: Ingel digestion

Lecture 5: Fundamentals of mass spectrometry

Lab session 1.1: Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR

Lab session 1.2: Demonstration of Q-TOF MS technology

Week 2: Basics of Mass Spectrometry

Lecture 6: Chromatography technologies

Lecture 7: Liquid chromatography

Lecture 8: Mass spectrometry: Ionization sources

Lecture 9: Mass spectrometry: Mass analyzers

Lecture 10: MALDI sample preparation and analysis

Lab session 2.1: Sample preparation: tissue sample preservation technology

Lab session 2.2: Targeted proteomics using triple quadrupole mass spectrometry

Week 3: Quantitative Proteomics

Lecture 11: Introduction to quantitative proteomics

Lecture 12: Hybrid mass spectrometry configurations

Lecture 13: SILAC: In vivo labeling

Lecture 14: iTRAQ: In vitro labeling

Lecture 15: TMT: In vitro labeling

Lab session 3.1: Mass spectrometry data analysis

Lab session 3.2: Targeted proteomics: parallel reaction monitoring

Week 4: Proteomics and Systems Biology

Lecture 16: Quantitative proteomics data analysis

Lecture 17: Proteomics and systems biology I

Lecture 18: Proteomics and systems biology II

Lecture 19: Proteomics applications

Lecture 20: Challenges in proteomics

Supplementary : Latest revolutions in omic technologies

Lab session 4.1: Protein-protein interaction using label-free biosensors

Lab session 4.2: SWATH-MS technology

Books and references

Nil.

Instructor bio

Prof. Sanjeeva Srivastava

IIT Bombay

Prof. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings.

Prof. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013].

These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay.

Prof. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society advance the proteomics knowledge for the benefits of global health.

Click here to view Faculty Profile: Prof. Sanjeeva Srivastava

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.

The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).

Date and Time of Exams: April 25, 2026 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.

Registration url: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.

Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 3 assignments out of the total 4 assignments given in the course.

Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

Please note that assignments encompass all types (including quizzes, programming tasks, and essay submissions) available in the specific week.

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Bombay .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team