Animal Transgenesis: Principles and Methods

By Prof. Himanshu Kumar | IISER Bhopal

Learners enrolled: 2791 | Exam registration: 616

ABOUT THE COURSE:

Transgenesis is the process of introducing changes into the genome of an organism to create a transgenic organism. Transgenic animals are valuable for fundamental studies of biological phenomena and have significant relevance in the development of highly specific therapeutics and biologics. The technology for creating transgenic mice has evolved rapidly, progressing from traditional recombination methods to modern CRISPR-Cas9-mediated techniques.

In this course, I will discuss the principles and methods of animal transgenesis, with a particular focus on mice

INTENDED AUDIENCE: Final year BS/BSc students studying subjects related to animal/human, BVSc, MBBS, MS/MSc students studying subjects related to animal/human, MVSc, and PhD students.

PREREQUISITES: For doing the course is that students/candidates should have good basic knowledge of "Molecular Biology", "Biochemistry", "Genetic Manipulation/Genetic engineering", and "Immunology".

INDUSTRY SUPPORT: Any biotechnology industry

Summary

Course Status :	Ongoing
Course Type :	Elective
Language for course content :	English
Duration :	4 weeks
Category :	Biological Sciences & Bioengineering Biotechnology Biosciences
Credit Points :	1
Level :	Postgraduate
Start Date :	16 Feb 2026
End Date :	13 Mar 2026
Enrollment Ends :	16 Feb 2026
Exam Registration Ends :	27 Feb 2026
Exam Date :	19 Apr 2026 IST
NCrF Level	4.5 — 8.0

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1: Introduction of transgenesis; Brief History of transgenesis; why we need transgenesis? Model organisms for transgenesis and application of various transgenic animals in basic discoveries and translational research in development of clinical general/precision therapeutics; Practical Application of 3Rs (Replace, Reduce, Refine) in Mouse

Week 2: General principles of transgenesis in Mouse; Tools and Technique for creation of transgenic mice; creation and methods for gene overexpressing mice; Methods for animal/mammalian cell culturing;

Week 3: Tools and Technique for creation of transgenic mice using homologous recombination method. Creation of Gene-deficient/knockout mice using homologous recombination method. Creation of conditional or cell-type specific- or tissue type-specific gene-deficient mice.

Week 4: Tools and Technique for creation of transgenic mice using CRISPR-Cas9 method. Creation of Gene-deficient mice. Creation of conditional or cell-type specific- or tissue type-specific gene-deficient mice. Creation of gene inserted mice.

Books and references

1. Freshney’s Culture of Animal Cells
2. Transgenesis Method and Protocols
3. Transgenesis Techniques
4. I have expertise in making transgenic mice so I will prepare my own materials.

Instructor bio

Prof. Himanshu Kumar

IISER Bhopal

Prof. Himanshu Kumar involve in teaching and research in immunology at the Department of Biological Sciences, IISER Bhopal. He is working in Innate Immunobiology. He obtained Ph.D. from Graduate School of Medicine, Osaka University, Japan (2008) and he created several kinds of transgenic mouse during his Ph.D. course and awarded as Excellent Ph.D. Student of Medical School. Prof. Kumar is a recipient of the Ramanujan National Fellowship of the DST in 2010. He is also Guest Professor of Immunology Frontier Research Centre, a top Immunology Institution in the world. He is working as “Editor-in-Chief” of “International Reviews of Immunology.”

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.

The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).

Date and Time of Exams: April 19, 2026 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.

Registration url: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.

Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 3 assignments out of the total 4 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

Please note that assignments encompass all types (including quizzes, programming tasks, and essay submissions) available in the specific week.

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IISER Bhopal .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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