Week 1: Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods, Nucleic Acid Structure and Function, DNA Replication and Repair, RNA Transcription and Processing, Protein Synthesis from mRNA-Translation
Week 2: Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals, RT PCR and qPCR, Modifications of PCR-Hot start, Touch down, nested PCR, Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR, Methylation-specific PCR (MSP), Digital Droplet PCR-modern implications, PCR-based mutation analysis
Week 3: Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform, Application of NGS in Molecular Diagnostics, Clinical Interpretation of NGS Data, Whole genome vs Whole exome sequencing, Targeted gene panels, NGS library preparations)
Week 4: Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray, FISH (Fluorescence in situ Hybridization), Serial analysis of gene expression, RNA sequencing, Tiling array, DNA protein interaction- chromatin immune precipitation.
Week 5: Techniques of Gene Manipulation: RNA interference and detection methods, Recombinant DNA Technology, CrispR-CAS9 technology, Epigenetics and diseases, DNA methylation analysis.
Week 6: Proteomics: Clinical Applications Overview of proteomics techniques and workflows, Protein separation techniques-brief discussion of gel electrophoresis and
chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis.
Week 7: Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA), Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools, Luminex multiplex assays and its application in biomarker analysis.
Week 8: Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing, POC Molecular diagnostics for infectious diseases, Molecular diagnostics in Hospital acquired infections.
Week 9: Molecular Diagnostics in Cancer Management Cancer markers, Liquid biopsies in cancer detection, circulating Tumour DNA (ctDNA) analysis, Monitoring treatment response with molecular diagnostics, Molecular diagnostics in targeted therapy, Digital PCR, Molecular diagnostics quality control
Week 10: Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases, Non-Invasive Prenatal testing (NIPT) and reproductive genetics, Molecular diagnostics in rare genetic disorders, Pharmacogenomics and Personalized Medicine, genetic counselling and patient education.
Week 11: Molecular Diagnostics in Medicine Molecular diagnostics in Metabolic disease, Molecular diagnostics in Neurodegenerative disease, Molecular diagnostics in Respiratory, Molecular diagnostics in Gastrointestinal disorders, Molecular diagnostics in Endocrine disorders, Molecular diagnostics in Autoimmune disorders, Molecular diagnostics in Cardiovascular diseases, Molecular diagnostics in Transplantation diseases
Week 12: Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics , Ethical Concerns in Molecular Diagnostics,Microfluidics and Lab-on-chip in molecular diagnostics, AI and ML in molecular diagnostics, Nanotechnology based molecular diagnostics, Single cell Analysis, Integration of Multi-omics Data.
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