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Comprehensive Molecular Diagnostics and Advanced Gene Expression Analysis

By Prof. Aritri Bir, Prof. Arindam Ghosh   |   IIT Kharagpur
Learners enrolled: 1305   |  Exam registration: 391
ABOUT THE COURSE:
Explore the frontiers of medical science with our course on "Molecular Diagnostics and Advanced Gene Expression Analysis." Uncover the historical evolution and advantages of molecular techniques, delve into the intricacies of nucleic acids, and master cutting-edge methodologies like PCR, NGS, and CRISPR-Cas9. Discover their clinical applications in infectious diseases, cancer, genetics, and more. Navigate the ethical considerations and future trends shaping the field. Join us to revolutionize your understanding of modern healthcare, molecular bioinformatics and precision medicine.

INTENDED AUDIENCE:
Medical Laboratory Professionals: This includes medical technologists, clinical laboratory scientists, and technicians who perform diagnostic tests. A course in molecular diagnostics can enhance their skills and knowledge in using advanced molecular techniques for disease diagnosis.

Medical Practitioners: Doctors, especially those specializing in fields like oncology, infectious diseases, genetics, and personalized medicine, can benefit from understanding molecular diagnostic techniques to make more informed treatment decisions.

Research Scientists: Researchers in academia, pharmaceuticals, and biotechnology can use molecular diagnostics to study diseases, develop new therapies, and conduct clinical trials. A course can equip them with the necessary skills to incorporate molecular diagnostics into their work.

Genetic Counselors: Professionals who provide information and support to individuals and families with genetic disorders can expand their expertise by learning about molecular diagnostics and its applications.

Pharmaceutical Professionals: Those working in the pharmaceutical industry can benefit from understanding molecular diagnostics for drug development, pharmacogenomics, and monitoring treatment responses.

Public Health Officials: Professionals involved in disease surveillance, outbreak investigation, and epidemiology can utilize molecular diagnostics for accurate and rapid disease detection.

Biomedical Engineers: Individuals working in the field of medical device development and instrumentation can apply molecular diagnostics principles to design innovative diagnostic tools.

Healthcare Administrators: Administrators responsible for managing healthcare facilities and services can benefit from understanding the potential impact of molecular diagnostics on patient care and resource allocation.

Graduate and Postgraduate Students: Students pursuing degrees in medical sciences, biotechnology, genetics, microbiology, or related fields can enhance their knowledge and increase their employability by taking a course in molecular diagnostics.

Entrepreneurs and Innovators: Individuals interested in starting healthcare-related businesses, such as diagnostic laboratories or biotech startups, can gain valuable insights into the field through a molecular diagnostics course.

PREREQUISITES: Basics in molecular biology, Foundations in cell biology, biochemistry and biotechnology

INDUSTRY SUPPORT: Healthcare Sector/institutions, Biotechnology and Biosciences based companies, Research institutions, Pharmaceutical companies, diagnostic laboratories, Medical device manufacturers, Genetic testing laboratories.
Summary
Course Status : Completed
Course Type : Core
Language for course content : English
Duration : 12 weeks
Category :
  • Multidisciplinary
  • Bioengineering
  • Computational Biology
Credit Points : 3
Level : Undergraduate/Postgraduate
Start Date : 22 Jan 2024
End Date : 12 Apr 2024
Enrollment Ends : 05 Feb 2024
Exam Registration Ends : 16 Feb 2024
Exam Date : 28 Apr 2024 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.


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Course layout

Week 1:  Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods, Nucleic Acid Structure and Function, DNA Replication and Repair, RNA Transcription and Processing, Protein Synthesis from mRNA-Translation

Week 2: Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals, RT PCR and qPCR, Modifications of PCR-Hot start, Touch down, nested PCR, Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR, Methylation-specific PCR (MSP), Digital Droplet PCR-modern implications, PCR-based mutation analysis

Week 3: Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform, Application of NGS in Molecular Diagnostics, Clinical Interpretation of NGS Data, Whole genome vs Whole exome sequencing, Targeted gene panels, NGS library preparations)

Week 4: Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray, FISH (Fluorescence in situ Hybridization), Serial analysis of gene expression, RNA sequencing, Tiling array, DNA protein interaction- chromatin immune precipitation.

Week 5: Techniques of Gene Manipulation: RNA interference and detection methods, Recombinant DNA Technology, CrispR-CAS9 technology, Epigenetics and diseases, DNA methylation analysis.

Week 6: Proteomics: Clinical Applications Overview of proteomics techniques and workflows, Protein separation techniques-brief discussion of gel electrophoresis and
chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis.

Week 7: Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA), Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools, Luminex multiplex assays and its application in biomarker analysis.

Week 8: Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing, POC Molecular diagnostics for infectious diseases, Molecular diagnostics in Hospital acquired infections.

Week 9: Molecular Diagnostics in Cancer Management Cancer markers, Liquid biopsies in cancer detection, circulating Tumour DNA (ctDNA) analysis, Monitoring treatment response with molecular diagnostics, Molecular diagnostics in targeted therapy, Digital PCR, Molecular diagnostics quality control

Week 10: Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases, Non-Invasive Prenatal testing (NIPT) and reproductive genetics, Molecular diagnostics in rare genetic disorders, Pharmacogenomics and Personalized Medicine, genetic counselling and patient education.

Week 11: Molecular Diagnostics in Medicine Molecular diagnostics in Metabolic disease, Molecular diagnostics in Neurodegenerative disease, Molecular diagnostics in Respiratory, Molecular diagnostics in Gastrointestinal disorders, Molecular diagnostics in Endocrine disorders, Molecular diagnostics in Autoimmune disorders, Molecular diagnostics in Cardiovascular diseases, Molecular diagnostics in Transplantation diseases

Week 12: Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics , Ethical Concerns in Molecular Diagnostics,Microfluidics and Lab-on-chip in molecular diagnostics, AI and ML in molecular diagnostics, Nanotechnology based molecular diagnostics, Single cell Analysis, Integration of Multi-omics Data.

Books and references

1. Molecular Cloning: A Laboratory Manual by David W. Russell and Joseph Sambrook
2. "Molecular Diagnostics: Fundamentals, Methods, & Clinical Applications" by Lela Buckingham and Maribeth L. Flaws
3."PCR (The Basics)" by Michael L. Mader
4. "Real-Time PCR: Advanced Technologies and Applications" by Nick A. Saunders and Martin A. Lee
5. "Next-Generation Sequencing: Translation to Clinical Diagnostics" by Alireza Heravi-Moussavi
6. "Genetic Testing and Molecular Biomarkers" by George P. Patrinos and William B. Coleman
7. "Essentials of Genomic and Personalized Medicine" by Geoffrey S. Ginsburg and Huntington F. Willard
8. "Bioinformatics for Beginners: Genes, Genomes, Molecular Evolution, Databases and Analytical Tools" by SupratimChoudhuri
9. "Genomic Medicine: Principles and Practice" by Dhavendra Kumar
10. "Pharmacogenomics: Challenges and Opportunities in Therapeutic Implementation" by Urs A. Meyer and FolefacAminkeng
11. "Cancer Genomics: From Bench to Personalized Medicine" by Graham Dellaire and Jason N. Berman
12. "Introduction to Genetic Analysis" by Anthony J.F. Griffiths, Susan R. Wessler, Sean B. Carroll, John Doebley
13. "Medical Genetics: A Core Text with Integrated Cases" by Lynn B. Jorde, John C. Carey, Michael J. Bamshad
14. "Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory" by Wayne W. Grody and Robert M. Nakamura
15. "Molecular Pathology in Clinical Practice" by Debra G.B. Leonard
16. "Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing" by William B. Coleman and Gregory J. Tsongalis

Instructor bio

Prof. Aritri Bir

IIT Kharagpur
Prof. Aritri Bir is a MBBS (Hons), MD Biochemistry, DNB Biochemistry (Gold Medalist), MNAMS Assistant Professor at the Department of Biochemistry, Dr. B.C. Roy Multispecialty Medical Research Centre, IIT Kharagpur. Over 8 years of post PG teaching experience as a medical faculty in different undergraduate and post Graduate Medical Colleges while teaching Post Graduate (MD Biochemistry) as well as Undergraduate Medical (MBBS, Nursing and DMLT) Students. Trained in Revised Basic Course workshop as well as in Advanced Course in Medical Education. Faculty member of Competency Based Medical Education, Biochemistry group by Medical Education Unit, India.


Prof. Arindam Ghosh

Prof. Arindam Ghosh is a MBBS (Hons), MD Biochemistry, DNB Biochemistry (Gold Medalist), MNAMS Assistant Professor at the Department of Biochemistry, Dr. B.C. Roy Multispecialty Medical Research Centre, IIT Kharagpur. Over 8 years of post PG teaching experience as a medical faculty in different undergraduate and post Graduate Medical Colleges while teaching Post Graduate (MD Biochemistry) as well as Undergraduate Medical (MBBS, Nursing and DMLT) Students. Trained in Revised Basic Course workshop as well as in Advanced Course in Medical Education. Faculty member of Competency Based Medical Education, Biochemistry group by Medical Education Unit, India.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 28 April 2024 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Kharagpur .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team


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