NMR spectroscopy for Chemists and Biologists

By Prof. Ashutosh Kumar, Prof. R. V Hosur | IIT Bombay

Learners enrolled: 751 | Exam registration: 83

ABOUT THE COURSE :

This course will start with Basic principles of NMR, walk through the analysis of spectra and demonstrate the application of multidimensional NMR spectroscopy in Chemistry and structural Biology.

INTENDED AUDIENCE: M. Sc./ PhD and Scientists working in Pharma and Biophrma Industries

PREREQUISITES: Under graduate level understanding of Physics and Mathematics

INDUSTRY SUPPORT : Biocon, Wockhardt, Aurobindo Biopharma etc

Summary

Course Status :	Completed
Course Type :	Elective
Language for course content :	English
Duration :	12 weeks
Category :	Chemistry
Credit Points :	3
Level :	Postgraduate
Start Date :	22 Jul 2024
End Date :	11 Oct 2024
Enrollment Ends :	05 Aug 2024
Exam Registration Ends :	16 Aug 2024
Exam Date :	27 Oct 2024 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1 :

Nuclear Spin and Magnetic Moments
Nuclear Spins in a Magnetic Field Spin
Lattice Relaxation Spin temperature Resonance
Absorption of Energy and The NMR Experiment Resonance
Absorption of Energy and The NMR Experiment Kinetics of Resonance Absorption

Week 2 :

Selection Rules and Line widths
Bloch equations
More about relaxation
More about relaxation Sensitivity

Week 3 :

Instruction to operator Algebra
Chemical Shift
Anisotropy of chemical shifts Learning spectral simulation

Week 4 :

Factors Influencing Isotropic Chemical shifts: Spin Spin Coupling
Analysis of NMR spectra of molecules
Learning spectral simulation

Week 5 :

Dynamic Effects in the NMR spectra : Two site exchange
Collapse of spin multiplets
Conformational Averaging of J- values
Analysis of NMR spectra of molecules with J Values

Week 6 :

Principles of Fourier transform
NMR Theorems on Fourier transforms
Practical aspects of recording FTNMR spectra
Free Induction Decay (FID) and the spectrum
Pulse repetition rate
Folding of signals
Acquisition time and the resolution
Data processing in FT
NMR Learning of Data processing

Week 7 :

Dynamic range in FTNMR and solvent suppression
The Nuclear Overhauser Effect - Experimental Schemes,Advanced Treatment
Steady state NOE and Transient NOE

Week 8 :

Spin Echo Uncoupled spins
Spin Echo Coupled spins
Spin-lattice relaxation
Spin-spin relaxation
Polarization transfer SPT and INEPT spectral simulation

Week 9 :

Density matrix, Elements of Density Matrix, Time evolution of density operator
Time evolution of density operator
Product operator formalism

Week 10 :

Segmentation of the time axis
Two dimensional NMR 2D
Fourier Transformation in NMR
Peak shapes in 2D spectrum
Quadrature detection in two-dimensional NMR

Week 11 :

2D- resolution/ separation experiments
Two-dimensional correlation experiments COSY, TDQ-COSY etc TOCSY

Week 12 :

2D NOESY, 2D ROESY, Heteronuclear COSY,
The HETCOR pulse sequence HSQC

Books and references

1. NMR Spectroscopy: Basic Principles, Concepts and Applications in Chemistry by Herald Gunther

2. NMR spectroscopy by RK Harris

3. Protein NMR spectroscopy: Principles and Practice Arthur G. Palmer, III, John Cavanagh, Nicholas J. Skelton, and Wayne J. Fairbrother

4. Spin Dynamics by M.H. Levitt

Instructor bio

Prof. Ashutosh Kumar

IIT Bombay

Prof. Kumar is an Associate Professor in the Department of Biosciences and Bioengineering. His area of research is NMR based structural biology. Prof. Kumar develop and apply NMR methods to understand protein structure and dynamics.

Prof. R. V Hosur

Prof. Hosur is Distinguished Visiting Professor in the Department of Biosciences and Bioengineering, IIT Bombay. Prior to this, he was senior Professor in Tata Institute of Fundamental Reseach, Mumbai

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 27 October 2024 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Bombay.It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team