Proteins and Gel-Based Proteomics

By Prof. Sanjeeva Srivastava | IIT Bombay

Learners enrolled: 893 | Exam registration: 151

ABOUT THE COURSE

This course introduces to the basic biology of proteins and gel-based proteomics. To obtain better understanding of cellular processes & regulation, there has been an increasing interest in studying proteomics. Proteomics techniques aim to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. These proteomic techniques typically aim to elucidate the expression, interaction, and cellular function of proteins.

The course will provide the basic understanding of amino acids and proteins. It would also provide detailed information on gel-based proteomic techniques such as SDS-PAGE and 2-DE. Some of the limitation of 2-DE has been overcome by advanced Difference gel electrophoresis (DIGE) technique, which will be discussed in this course. Protein identification using MALDI-TOF/TOF mass spectrometry will also be discussed. 2-DE in combination with mass spectrometry has become very powerful tool for proteomics applications and students can learn these tools by attending this course.

INTENDED AUDIENCE

Core course

All UG and PG students can attend this course.

It would be applied to B.Sc., M.Sc. and Ph.D

PRE-REQUISITES

B.Sc. Or M.Sc.

The target audiences of this course are required to have a basic biology and biochemistry background.

Summary

Course Status :	Completed
Course Type :	Core
Language for course content :	English
Duration :	4 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	1
Level :	Undergraduate/Postgraduate
Start Date :	22 Jul 2024
End Date :	16 Aug 2024
Enrollment Ends :	05 Aug 2024
Exam Registration Ends :	16 Aug 2024
Exam Date :	22 Sep 2024 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week-1: Basics of amino acids and proteins

L1. Introduction to amino acids

L2. Introduction to proteins

L3. Protein folding & misfolding

L4. Protein purification techniques

L5. Introduction to proteomics

Lab session-1.1 Demonstration of protein-protein interaction using label-free biosensors

Week-2: Gel-based proteomics and Sample Preparation

L6. Systems biology and proteomics

L7. Sample preparation and pre-analytical factors

L8. Sample preparation: Pre-analytical factors (contd.)

L9. Sample preparation: Protein extraction and quantification

L10. One-dimensional electrophoresis

Lab session-2.1 Sample preparation: Tissue sample preservation technology

Lab session-2.2 Protein/peptide pre-fractionation using OFF-GEL FRACTIONATOR

Week-3: Two-dimensional gel electrophoresis (2-DE)

L11. 2-DE: Rehydration, IEF & Equilibration

L12. 2-DE: Second dimension, staining & destaining

L13. 2-DE: Gel analysis

L14. 2-DE: Applications

L15. 2-DE: Applications (contd.) & Challenges

Lab session-3.1 Demonstration of gel analysis using IQTL software

Week-4: Difference in gel electrophoresis (DIGE) & Mass Spectrometry

L16. 2D-DIGE: Basics

L17. 2D-DIGE: Data analysis

L18. 2D-DIGE: Applications

L19. Protein identification using MALDI-TOF/TOF

L20. Proteomics experiment data analysis & challenges

Lab session-4.1 Demonstration of Q-TOF MS technology

Instructor bio

Prof. Sanjeeva Srivastava

IIT Bombay

Prof. Sanjeeva Srivastava Dr. Sanjeeva Srivastava is a Professor and group head of proteomics laboratory at the Indian Institute of Technology, Bombay. He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings. He joined IIT Bombay in 2009 as an Assistant Professor and currently working as Professor. Current research in his group centers on biomarker and drug target discovery and deciphering the protein interaction networks in complex human diseases (gliomas) and infectious diseases (malaria) using high throughput proteomics, protein microarrays and mass spectrometry. Dr. Srivastava is an active contributor to global proteomics science and innovation. He serves on the Executive Council of Human Proteome Organization (HUPO) and Proteomics Society, India (PSI). He has organized three successful international conferences & workshops at IIT Bombay PSI-2014, Targeted Proteomics International Symposium in 2015 and 2018. He has published four special issues as editor, Proteomics in India for Journal of Proteomics; Proteomics Research in India for Nature India, Protein Arrays for Proteomics and Neglected Tropical Infectious Diseases for Proteomics Clinical Applications. Having an extensive teaching experience at IITB and experience of conducting proteomics courses at CSHL, New York provided him with the background to increase proteomics education for the global community. One of his special contributions has been the development of e-learning resources (MOOC mass spectrometry and interactomics courses; Virtual Proteomics Laboratory). He has made first ever proteomics documentaries Proteomics: Translating the Code of Life and Human Proteome Project (HPP). He has directed HUPO Perspective in Proteomics video interview series, which is hosted on HUPO website. Recently we have signed a MOU on clinical proteogenomics cancer research with National Cancer Institute, along with Tata Memorial Centre and India has now become 12th country to join the International Cancer Proteogenome Consortium (ICPC). Dr. Srivastava continues to develop proteomics & omics science and innovation together with and for the next generation of keen students, researchers and the research and education commons in Asia and global OMICS community.

Click here to view Faculty Profile: http://www.bio.iitb.ac.in/~sanjeeva/

About the Instructor: https://youtu.be/sb4faypvWwk

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 22 September 2024 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 3 assignments out of the total 4 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Bombay .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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