Molecular Biology

By Prof. Vishal Trivedi | IIT Guwahati

Learners enrolled: 4891 | Exam registration: 1050

ABOUT THE COURSE:
In the current MOOCs course, I have put effort to discuss different aspects of molecular biology for engineer graduate students as well as MTech students. Biological processes are controlled by different types of molecules such as cell signaling, replication, transcription, protein expression and development. Apart from these aspects, molecular biology theory leads to the development of several diagnostic methods and recombinant DNA technology. At the end of the course, student could be able to understand following aspects;
1. Basics understanding of central dogma of molecular biology involving replication, transcription and translation. In addition, we will discuss about structure and function of cells, their manipulation and exploitation using molecular biology tools.
2. Discussion about mutagenesis and repair mechanism. Approaches for Genome Editing and gene silencing.
3. At the end we will discuss the role of molecular biology to develop different types of techniques, cloning and over-expression of foreign protein in expression system.

INTENDED AUDIENCE: UG/PG

INDUSTRY SUPPORT: Biocon https://www.biocon.com/
Jubilant Life Sciences www.jubl.com/
Shantha Biotechnics Ltd
Panacea Biotec
Serum institute of india

Summary

Course Status :	Completed
Course Type :	Core
Duration :	12 weeks
Category :	Biological Sciences & Bioengineering Biosciences
Credit Points :	3
Level :	Undergraduate
Start Date :	22 Jan 2024
End Date :	12 Apr 2024
Enrollment Ends :	05 Feb 2024
Exam Registration Ends :	16 Feb 2024
Exam Date :	21 Apr 2024 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1:Basics of Biological System (Part 1)
Lecture 1: Cellular structure (Part I)
Lecture 2: Cellular structure (Part II)
Lecture 3: Cellular structure (Part III)
Week 2 : Basics of Biological System (Part 2)
Lecture 4: Cellular Metabolism
Lecture 5: Cell Cycle and control
Lecture 6: Program Cell Death
. Week 3: Basics of Biomolecules
Lecture 7: DNA
Lecture 8: RNA
Lecture 9: Protein
Week 4: Central Dogma of Molecular Biology
Lecture 10: Genetic Material (Part 1)
Lecture 11: Genetic Material (Part 2)
Lecture 12: Central Dogma of Molecular Biology
Week 5: Replication
Lecture 13: Replication (Part 1)
. Lecture 14: Replication (Part 2)
Lecture 15: Mutagenesis and repair mechanism
Week 6: Transcription
Lecture 16: Transcription in Prokaryotes
Lecture 17: Transcription in Eukaryotes
Lecture 18: Post-transcriptional Modifications
. Week 7: Translation
Lecture 19: Translation in Prokaryotes
Lecture 20: Translation in Eukaryotes
Lecture 21: Post-translational Modifications
Lecture 22: Gene control mechanisms
Week 8: Molecular Techniques (Part 1)
Lecture 23: SouthernBlotting
Lecture 24: Northern Blotting
Lecture 25: Western Blotting
Week 9: Molecular Techniques (Part 2)
Lecture 26: Polymerase chain reaction (Part 1)
Lecture 27: polymerase chain reaction (Part 2)
Lecture 28: Real-Time PCR
. Week 10: Molecular Cloning
Lecture 29: Cloning of gene
Lecture 30: DNA delivery
. Lecture 31: Screening of cloned DNA
Week 11: Genome Editing
Lecture 32: Genome Editing (Part 1)
. Lecture 33: Genome Editing (Part 2)
Lecture 34: Genome Editing (Part 3)
Week 12: Application of Molecular Biology
Lecture 35: Application of Molecular Biology (Part 1)
Lecture 36: Application of Molecular Biology (Part 1)
Lecture 37: Summary and Conclusions

Books and references

Texts:
1. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, Molecular Biology of Cell, 7th Ed., Garland Publishing, 2022.
2. H. Lodish, A. Berk, S. L. Zipursky, M. P. Scott and J. Darnell, Molecular Cell Biology, 9th Ed., W. H. Freeman & Co., 2021.
3. B. Lewin, Genes XII, International Edition, Pearson Education, 2017.
4. B. R. Glick and J. J. Pasternak, Molecular Biotechnology: Principles and Applications of Recombinant DNA, 3rd Ed., ASM Press, 2003.
5. R. M. Twyman, S. B. Primrose and R. W. Old, Principles of Gene Manipulation, Blackwell Science, 7th edition, 2006.

Instructor bio

Prof. Vishal Trivedi

IIT Guwahati

Prof. Trivedi did his Ph.D. from Central Drug Research Institute, Lucknow in the field of Structural Biology. From his postdoctoral research at the Department of Molecular and Cellular Biology, Harvard University and Molecular Oncology Research Institute, Tufts University, Boston, USA, he gained extensive research experience in the field of cell biology, intracellular signal transduction, and immunology. Currently, his laboratory at Department of Biosciences and Bioengineering has an active group working and exploring questions related to malaria parasite biochemistry, the role of novel proteins, development of anti-malarial agents, and lastly understanding factors playing a crucial role in immunomodulation and host pathology in different organs

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.

The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).

Date and Time of Exams: 21 April 2024 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.

Registration url: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.

Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Guwahati .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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