Enzyme Sciences and Technology

By Prof. Vishal Trivedi   |   IIT Guwahati
Learners enrolled: 1623
In the current MOOCs course I have put effort to discuss different aspects of enzymology for engineer graduate students as well as MSc students. Enzyme play pivotal role in running different types of metabolic reactions, converting toxic substances into non-toxic products and they are essential for running several physiological processes.
1. Basics understanding of enzyme structure and functions, its production through different types strategies, purification etc.
2. Understanding different approaches to study the interaction of substrate with enzymes, enzyme assay and kinetics.
3. Broad over-view of different approaches to design inhibitors against enzyme, understanding the enzyme-inhibitor kinetics and mode inhibition.
4. Role of enzyme in cell metabolism, physiology and application of enzymes.


PREREQUISITES: Basic Biochemistry

INDUSTRY SUPPORT: Biocon https://www.biocon.com/
Jubilant Life Sciences www.jubl.com/
Shantha Biotechnics LtdPanacea Biotec Dr. Reddy’s laboratories
Course Status : Completed
Course Type : Elective
Duration : 12 weeks
Category :
  • Biological Sciences & Bioengineering
Credit Points : 3
Level : Undergraduate
Start Date : 23 Jan 2023
End Date : 14 Apr 2023
Enrollment Ends : 06 Feb 2023
Exam Registration Ends : 17 Mar 2023
Exam Date : 29 Apr 2023 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1:  Introduction to Enzymes
Lecture 1: Introduction to Enzymes
Lecture 2: Basics of Enzyme
Lecture 3: Enzyme Classification (Part-I)
Lecture 4: Enzyme Classification (Part-II)
Lecture 5: Enzyme Nomenclature
Week 2: Structure of enzyme
Lecture 6: Enzyme Structure (Part 1) 
Lecture 7: Enzyme Structure (Part 2)
Lecture 8: Enzyme Structure (Part 3)
Week 3: Enzyme Production (Part 1)
Lecture 9: Cloning of Enzyme (Part 1)
Lecture 10: Cloning of Enzyme (Part 2)
Lecture 11: Over-expression in Host
Week 4: Enzyme Production (Part 2)
Lecture 12: Extraction of enzyme
Lecture 13: Purification Strategies (Part 1)
Lecture 14: Purification Strategies (Part 2)
Week 5: Enzyme Production (Part 3)
Lecture 15: Purification Strategies (Part 3)
Lecture 16: Purification Strategies (Part 4)
Lecture 17: Enzyme Characterization Approaches
Week 6: Enzyme catalyzed Biochemical reactions
Lecture 18: Enzyme Catalyzed reactions (Part 1: Carbohydrate Metabolism) 
Lecture 19: Enzyme Catalyzed reactions (Part 2: Lipid and Protein Metabolism) 
Lecture 20:  Enzyme Catalyzed reactions (Part 3: Detoxification)
Week 7: Enzyme-Substrate interaction
Lecture 21:Enzyme-Substrate interaction (Part 1: Spectroscopic approaches)
Lecture 22:Enzyme-Substrate interaction (Part 2: Isothermal Calorimetry)
Lecture 23:   Enzyme-Substrate interaction (Part 3: Surface plasma resonance)
Week 8: Enzyme assay system and Kinetics
Lecture 24: Enzyme assay system
Lecture 25: Enzyme Kinetics (Part 1)
Lecture 26:  Enzyme Kinetics (Part 2)
Week 9: Enzyme Inhibitor Designing
Lecture 27: Inhibitor designing (Part 1: Traditional approach)
Lecture 28: Inhibitor designing (Part 2: Modern approach)
Lecture 29: Inhibitor designing (Part 3: Computational approaches)
Week 10: Enzyme Inhibition kinetics
Lecture 30: Enzyme Inhibition kinetics (Part 1)
Lecture 31:Enzyme Inhibition kinetics (Part 2)
Lecture 32:  Enzyme Inhibition kinetics (Part 3)
Week 11: Enzyme Applications (Part 1)
Lecture 33: Enzymes in Industrial setup (Part 1)
Lecture 34: Enzymes in Industrial setup (Part 2)
Lecture 35: Enzymes in catalyzing chemical reactions
Week 12: Enzyme Applications (Part 2)
Lecture 36: Enzymes in medical field
Lecture 37: Enzymes in environment field
Lecture 38: Enzymes in drug discovery

Books and references

Text book:
1. Enzyme Kinetics: Behavior and analysis of rapid equlibraium and steady state enzyme systems. Irwin H SEGEL. ISBN: 978-0-471-30309-1.
2. Biochemistry. 5th edition, Berg JM, Tymoczko JL, Stryer L. publisher: W H Freeman
3. Biochemistry, 2nd edition Reginald Garrett and Charles Grisham
4. Cornish-Bowden, A., Fundamentals of Enzyme Kinetics (revised ed.), Portland Press (1995).
5. Nelson, David L. (David Lee), 1942-. Lehninger Principles of Biochemistry. New York :W.H. Freeman, 2005.
6. Voet, D., Voet, J. G., & Pratt, C. W. (2008). Fundamentals of biochemistry: Life at the molecular level. Hoboken, NJ: Wiley.
7. Tymoczko, Lubert Stryer,and LubertStryer. Biochemistry. New York: W.H. Freeman, 2002. Print.
Reference Book:
1. Enzymes Biocatalysis: Principles and Applications. ISBN 978-1-4020-8361-7.
2. Enzyme : Catalysis, kinetics and mechanisms. N.S. Punekar. ISBN 978-981-13-0784-3.
3. Enzymes 2nd Edition Biochemistry, Biotechnology, Clinical Chemistry by T Palmer P L Bonner
4. Color Atlas of Biochemistry 2nd edition by Jan Koolman and Klaus-Heinrich Roehm

Instructor bio

Prof. Vishal Trivedi

IIT Guwahati
Prof. Trivedi did his Ph.D. from Central Drug Research Institute, Lucknow in the field of Structural Biology. From his postdoctoral research at the Department of Molecular and Cellular Biology, Harvard University and Molecular Oncology Research Institute, Tufts University, Boston, USA, he gained extensive research experience in the field of cell biology, intracellular signal transduction, and immunology. Currently, his laboratory at Department of Biosciences and Bioengineering has an active group working and exploring questions related to malaria parasite biochemistry, the role of novel proteins, development of anti-malarial agents, and lastly understanding factors playing a crucial role in immunomodulation and host pathology in different organs

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 29 April 2023 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Guwahati .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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