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NMR spectroscopy for Structural Biology

By Prof. Ashutosh Kumar, Prof. R. V Hosur   |   IIT Bombay
Learners enrolled: 678
ABOUT THE COURSE:
This course will start with Basic principles of NMR, Discuss practical aspects of NMR spectroscopy, peptide NMR, Protein NMR, Nucleic acids NMR. We will teach with examples how to solve structure and measure dynamics using NMR.

PRE-REQUISITES: Under graduate level understanding of Physics and Mathematics, Basic course on NMR spectroscopy.

INTENDED AUDIENCE:
M.Sc./ PhD students & Scientist, and Scientists working in Biopharma Industries.

INDUSTRY SUPPORT:
Biocon, Sunpharma, Alembic, Bharat Biotech, Aurobindo Biopharma etc.
Summary
Course Status : Completed
Course Type : Elective
Language for course content :
Duration : 12 weeks
Category :
  • Chemistry
Credit Points : 3
Level : Postgraduate
Start Date : 25 Jul 2022
End Date : 14 Oct 2022
Enrollment Ends : 08 Aug 2022
Exam Date : 29 Oct 2022 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.


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Course layout

Week 1: Basics Principles of NMR; Nuclear Spin in static magnetic field
Week 2: Basics Principles continued; Chemical Shift; Factors Influencing Isotropic Chemical shifts
Week 3: NMR instrumentation and Peptide sample preparation; Overview of NMR instrument Magnet, transmitters etc
Week 4: Two-dimensional NMR And Practical Aspects for experiment; Two- dimensional correlation experiments COSY
Week 5: Peptide and Protein structure Structural features and parameters; Structural features and parameters
Week 6: Sample preparation for Protein NMR; Expression systems, Optimization of protein expression
Week 7: Multi-dimensional Heteronuclear NMR experiments; 2D Heteronuclea r NMR experiments
Week 8: Multi-dimensional Heteronuclear NMR experiments; Experiments for Backbone assignment
Week 9: Protein Structure determination from NMR data; Structure calculation methods and NMR parameters
Week 10: Protein dynamics from NMR data; Theory of spin relaxation in proteins
Week 11: Nucleic acids NMR experiments; Structural features and parameters for Nuclei acids
Week 12: DNA and structure determination from NMR data

Books and references

  1. Structural Biology practical NMR applications by Quincy Teng
  2. NMR of proteins and Nucleic acids by Kurt Wuthrich
  3. NMR spectroscopy by John Cavanagh, Arthur G. Palmer, III, Nicholas J. Skelton, Wayne J. Fairbrother

Instructor bio

Prof. Ashutosh Kumar

IIT Bombay
Prof. Kumar is an Associate Professor in the Department of Biosciences and Bioengineering. His area of research is NMR based structural biology. Prof. Kumar develop and apply NMR methods to understand protein structure and dynamics.


Prof. R. V Hosur

Prof. Hosur is Distinguished Visiting Professor in the Department of Biosciences and Bioengineering, IIT Bombay. Prior to this, he was senior Professor in Tata Institute of Fundamental Reseach, Mumbai

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 29 October 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Bombay .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team


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