Experimental Biochemistry

By Prof. Swagata Dasgupta, Prof. Soumya De   |   IIT Kharagpur
Learners enrolled: 1158
For undergraduate students in the discipline of biochemistry, molecular biology, chemistry and other related biological sciences, the biochemistry laboratory course has become an integral part. It is necessary for students to acquire skills in working with biomolecules in the laboratory. This course is designed to train students with the basic biochemistry laboratory techniques and also introduce some higher level concepts that will prepare them for future research and development projects. The course outline is given for each week. We will introduce each topic and give an overview of the topic and underlying theory. This will be followed by actual demonstration of the experiment. Weekly assignments will be provided and graded.

INTENDED AUDIENCE : CHEMISTRY, BIOCHEMISTRY & BIOTECHNOLOGY(Any student with a course in Biochemistry in their curriculum)
PRE-REQUISITES : Biochemistry desirablel
INDUSTRIES APPLICABLE : Biotech & Pharma companies
Course Status : Completed
Course Type : Core
Duration : 12 weeks
Category :
  • Chemistry
Credit Points : 3
Level : Undergraduate
Start Date : 24 Jan 2022
End Date : 15 Apr 2022
Enrollment Ends : 07 Feb 2022
Exam Date : 24 Apr 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1 : Basics of Experimental Biochemistry
Week 2 : Amino Acid Titration and pI determination
Week 3 : Spectroscopic techniques
Week 4 : Protein folding and denaturation studies
Week 5 : Chromatographic techniques
Week 6 : Gel electrophoresis of DNA and proteins
Week 7 : Isolation and characterization of proteins
Week 8 : Enzyme Kinetics
Week 9 : Isolation and characterization of DNA
Week 10 : Basics of rDNA technology
Week 11 : Protein ligand interactions
Week 12 : Immunoassay techniques

Books and references

Modern Experimental Biochemistry by Rodney F. Boyer.

Instructor bio

Prof. Swagata Dasgupta

IIT Kharagpur
Professor Swagata Dasgupta completed her B.Sc. (Hons) in Chemistry from Presidency College, Kolkata and her M.Sc. from IIT Kanpur. After a Post M.Sc. in Bioscience she obtained her Ph.D. from Rensselaer Polytechnic Institute, USA. She then joined IIT Kharagpur and initiated research in Protein Chemistry in the Department of Chemistry at IIT Kharagpur. Her areas of interest are protein–protein and protein-small molecule interactions and protein structure analyses. Professor Swagata Dasgupta has contributed to teaching and research at IIT Kharagpur for the past 25 years. She has over 150 publications in peer reviewed journals with over 3800 citations. Her interdisciplinary research activities in biophysical chemistry have led to several ongoing research collaborations. Over the years her laboratory has become an active center of research with generous support from funding agencies. Graduate students from her laboratory are well-placed both in India and abroad. Her teaching contributions range from classroom teaching, web-course development, introduction of new courses and teaching laboratories, development of curriculum, and initiation of research in a new area in the department. A video course in Biochemistry under the aegis of the National Program on Technology Enhanced Learning funded by the Ministry of Human Resource Development has received overwhelming response worldwide by both students and educators. She has given many popular lectures to young scholars and students and has been invited to inspire young women to science. She has served on many Interview boards and is also actively involved as a reviewer in many journals. Her contributions have been recognized by the award of the Bronze medal by the Chemical Research Society of India (CRSI) for 2016. She also received the Darshan Ranganathan Memorial Lecture Award of CRSI in 2013. She was inducted as a Fellow of the West Bengal Academy of Science and Technology, India in 2014. She is an elected Member of the National Academy of Science, Allahabad since 2013 and a Fellow of the Indian Academy of Sciences, Bangalore since 2018.

Prof. Soumya De

The overarching research goal of Dr. Soumya De's laboratory is to decipher the structural and dynamic principles that govern protein functions and their regulation. This is achieved using solution NMR spectroscopy and other biophysical methods that characterize the structure and dynamics of proteins.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 24 April 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup. It will have the logos of NPTEL and IIT Kharagpur .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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