Basics Of Fluorescence Spectroscopy

By Prof. Pratik Sen   |   IIT Kanpur
Learners enrolled: 1321

Fluorescence refers to light emission by substance after the absorption of electromagnetic radiation. Due to its environmental sensitivity and time resolved capability, fluorescence spectroscopy is now been widely applied to chemistry, biochemistry, biophysics, material science, forensic science, medicinal science, etc. Although fluorescence spectroscopy is used in many disciplines, this particular course is intended for the individuals willing to receive an in-depth introduction to the principles of fluorescence spectroscopy and its applications to chemistry and biology.


PREREQUISITES: BS/B.Sc., Knowledge of basic quantum mechanics

INDUSTRY SUPPORT: Systronics India Ltd., B/116-129, 1st floor, Supath-II Complex, Nr.Juna Wadaj Bus Terminus, Ashram Road, Ahmedabad - 380013; Premier Colorscan Instruments Pvt. Ltd.
Course Status : Completed
Course Type : Elective
Duration : 8 weeks
Category :
  • Chemistry
Credit Points : 2
Level : Undergraduate
Start Date : 24 Jan 2022
End Date : 18 Mar 2022
Enrollment Ends : 07 Feb 2022
Exam Date : 27 Mar 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1: Introduction to Fluorescence
Week 2: Instrumentation for Fluorescence Spectroscopy
Week 3: Time-Domain Lifetime Measurements
Week 4: Solvent and Environmental Effects
Week 5: Fluorescence Quenching
Week 6: Fluorescence Anisotropy
Week 7: Energy Transfer
Week 8: Single-Molecule Fluorescence

Books and references

Principles of Fluorescence Spectroscopy by J. R. Lakowicz, 3rd edition

Instructor bio

Prof. Pratik Sen

IIT Kanpur
Pratik Sen earned Ph.D. degree from Indian Association for the Cultivation of Science, Kolkata, India in 2006. Then he moved to RIKEN, Japan for his post-doctoral research work as a JSPS fellow. Presently he holds the position of associate professor in Department of Chemistry, IIT Kanpur, India. His current research interests include fluorescence spectroscopy, ultrafast laser spectroscopy, nonlinear spectroscopy and single molecule spectroscopy.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 27 March 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 6 assignments out of the total 8 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Kanpur .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

MHRD logo Swayam logo


Goto google play store