Cell Culture Technologies

By Prof. Mainak Das   |   IIT Kanpur
Learners enrolled: 5150
The course will be a short primer to understand how ‘animal cell culture technologies’ have strengthen the bio-medical research from basic research to the modern drug discovery. Animal cell culture was first performed in the very first decade of 19th century. Since then, tremendous development has taken place in this field. The lectures will help the researcher to appreciate the developments during last hundred years and will help them to independently set up cell culture laboratories. For non-biologist, it will be an informal way to demystify the intriguing routes of biomedical research where cell culture is a very ‘potent tool’.

INTENDED AUDIENCE : UG and PG students pursuing biology, biotechnology, zoology and bio-engineering.

PRE REQUISITE : Biology at standard 10th (Secondary school examination)

: Biomedical industries, Biotechnology industry, Drug -discovery industry
Course Status : Completed
Course Type : Elective
Duration : 8 weeks
Category :
  • Biological Sciences & Bioengineering
  • Bioengineering
  • Biosciences
Credit Points : 2
Level : Postgraduate
Start Date : 22 Aug 2022
End Date : 14 Oct 2022
Enrollment Ends : 22 Aug 2022
Exam Date : 29 Oct 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1: Introduction & biology of cultured cells
Week 2: Equipments, aseptic techniques, safety protocols
Week 3: Culture vessels & media development
Week 4: Serum-free medium development & sterilization
Week 5: Primary culture, secondary culture, cloning & selection
Week 6: Cell separation, characterization, differentiation & transformation
Week 7: Contamination, cryo-preservation & cyto-toxicity
Week 8: Organo-typic culture & specialized cell culture techniques

Books and references

  • Culture of Animal Cells by R Ian Freshney
  • Cell culture technology: Recent advances and future prospects (Euroscicon Meeting Reports Book 1) by Bruserud, Øystein and Astrid Englezou
  • Vertebrate Cell Culture II and Enzyme Technology: Volume 39 (Advances in Biochemical Engineering/ Biotechnology) by A.F. Bückmann and G. Carrea
  • Animal Cell Culture and Technology (The Basics) (Garland Science)) by Michael Butler
  • The Immortal Life of Henrietta Lacks by Rebecca Skloot

Instructor bio

Prof. Mainak Das

IIT Kanpur
Prof.Mainak Das is a faculty of IIT Kanpur India in the department of biological sciences & bioengineering since April 26 2010. He did his bachelors degree (1989-1994) in agriculture from College of Agriculture Indore. Thereafter he did his post graduate degree (1994-1997) in animal physiology from National Dairy Research Institute Karnal India. Following his post graduate studies, he worked as researcher in IISc Bangalore India (1997-1999), University of Neuchatel, Switzerland (1999-2000), University of Clemson, USA (2000-2004) and in University of Central Florida, USA (2004-2010). He did his doctoral studies from College of Medicine of University of Central Florida (2004-2008), while working as a full time employee of the university. He introduced the regular physiology course for the PG students in IIT Kanpur in 2011. He has wide interest in physiology, sensors, energy and bioelectronics and maintains an active research team at IT Kanpur, India. Prof. Das has been working on cell culture technologies, serum free medium development and defined cell culture systems for last 20 years. He has expertise in long term culturing of excitable cells. His doctoral thesis is a complex problem of modern cell culture technology, titled:‘Tissue Engineering The Motoneuron To Muscle Segment Of The Stretch Reflex Arc Circuit Utilizing Micro-fabrication,Interface Design And Defined Medium Formulation’.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 29 October 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 6 assignments out of the total 8 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Kanpur .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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