Principles Of Downstream Techniques In Bioprocess

By Prof. Mukesh Doble   |   IIT Madras
Learners enrolled: 822
The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing.The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied fieldThis course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.

PREREQUISITES :Basics of biochemical engineering and thermodynamics
Course Status : Completed
Course Type : Elective
Duration : 12 weeks
Category :
  • Biological Sciences & Bioengineering
  • Bioprocesses
Credit Points : 3
Level : Undergraduate/Postgraduate
Start Date : 25 Jul 2022
End Date : 14 Oct 2022
Enrollment Ends : 08 Aug 2022
Exam Date : 29 Oct 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1 : Lec-01 Introduction
Lec-02 Mass balance, Heat Balance, flow sheet
Lec-03 Costing
Week 2 : Lec-04 Costing (continued), Physical and chemical principles in Downs stream
Lec-05 Problems in Mass balance, flow sheet
Lec-06 Cell Breakage
Week 3 : Lec-07 Cell Breakage (continued)
Lec-08 Solid Liquid Separation
Lec-09 Solid Liquid Separation (continued)
Week 4 : Lec-10 Solid Liquid separation-problems
Lec-11 Pre-treatment and Filters
Lec-12 Adsorption
Week 5 : Lec-13 Adsorption (continued)
Lec-14 Adsorption (continued)
Lec15 Adsorption (continued)
Week 6 : Lec-16 Liquid-Liquid Extraction
Lec-17 Liquid-Liquid extraction (continued)
Lec-18 Liquid-Liquid extraction (continued)
Week 7 : Lec-19 Liquid-Liquid extraction (continued)
Lec-20 Reversed micellar and aqueous two phase extraction
Lec-21 Membranes
Week 8 : Lec-22 Membranes (continued)
Lec-23 Membranes (continued)
Lec-24 Membranes (continued)
Week 9 : Lec-25 Precipitation
Lec-26 Chromatography
Lec-27 Chromatography (continued)
Week 10 : Lec-28 Chromatography (continued)
 Lec-29 Chromatography (continued)
 Lec-30 Chromatography (continued)
Week 11:  Lec-31 Chromatography (continued)
 Lec-32 Chromatography (continued)
 Lec-33 Crystallisation
Week 12 : Lec-34 Drying
 Lec-35 Drying and Distillation
 Lec-36  Future trends, Summary of the course

Books and references

  1. Belter, P.A. and Cussler, E.L. Hu, W.S (1988), Bioseparation: Downstream processing for Biotechnology, Wiley, New York.
  2. Ladisch, M.R., (2001), Bioseparation Engineering: Principles, Practice and Economics, Wiley, Interscience.

Instructor bio

Prof. Mukesh Doble

IIT Madras
Mukesh Doble: Professor at the department of Biotechnology at IIT Madras. Has previously worked in Imperial chemical Industries(ICI) and General Electric(GE) for 20 years . Areas of research are Biomaterials, Biopolymers and Drug design. Published 270 papers and 10 books and filed 10 patents (including two US). Has delivered on line video courses in Downstream processes and Biostatistics.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 29 October 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Madras .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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