Genome Editing and Engineering

By Prof. Utpal Bora | IIT Guwahati

Learners enrolled: 1675

ABOUT THE COURSE:
This course is designed for UG & PG students, research scholars and young scientists to introduce them to basics and applications of genome editing and engineering. The course starts with understanding of basic organization and structure of genome. It gives a brief overview on different DNA strand breaks and their repair mechanism. It introduces learners to theoretical basics of genetic engineering and discusses it’s limitations in tackling genetic problems of animals and plants. The key concepts of Genome editing are discussed thoroughly with emphasis on the major genome editing tools ZFN, TALEN and CRISP/Cas9. It discusses the biochemical basis of development of the genome editing tools, their design and their applications in various genetic conditions. It also discusses the scope and prospect of using these technologies in solving major genetic disease of human. The learners will also be acquainted with the ethical concerns associated with applications of genome editing and engineering in germline.

INTENDED AUDIENCE: UG/PG/PhD/Scientist in industry

PREREQUISITES: 10+2 with Biology and Chemistry

Summary

Course Status :	Completed
Course Type :	Elective
Duration :	12 weeks
Category :	Biological Sciences & Bioengineering Biosciences
Credit Points :	3
Level :	Undergraduate/Postgraduate
Start Date :	25 Jul 2022
End Date :	14 Oct 2022
Enrollment Ends :	08 Aug 2022
Exam Date :	29 Oct 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1:Introduction to genetics and genetic engineering

Week 2:Breakage and Repair Of Genomic DNA

Week 3:Recombination

Week 4:Targeted genetic modification

Week 5:Zinc Finger Nuclease (ZFN) Technology

Week 6:Transcription activator-like effector nuclease (TALEN) Technology

Week 7:Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology

Week 8:Applications of genome editing in treating human diseases

Week 9:Genome engineered Disease modeling

Week 10:Engineered immune cells for cancer therapy

Week 11:Personalized therapy; Challenges: safety and specificity

Week 12:Ethical concerns: Germ line gene editing

Books and references

Harber , J. E., Genome Stability: DNA Repair and Recombination , Garland Science, 2013.
Yamamoto, T. , Targeted Genome Editing Using Site-Specific Nucleases, Springer, 2015.
Zlatanova, J. and Holde, K. van, Molecular Biology: Structure and Dynamics of Genomes and Proteomes. Garland Science, 2015.
Yamamoto, T.(Ed.), Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System , Springer 2015.
Barrangou , R. and Oost, J. van der, CRISPR-Cas Systems: RNA-mediated Adaptive Immunity in Bacteria and Archaea , Springer, 2013.
Addgene, CRISPR 101:A Desktop Resource , January 2016
Alberts , B. , Johnson , A., Lewis , J., Morgan, D., Raff, M., Roberts, K.and Walter, P., Molecular Biology of the Cell, 6^th Edn., Garland Science, 2014.

Instructor bio

Prof. Utpal Bora

IIT Guwahati

Prof. Utpal Bora is Professor in Department of Biosciences and Bioengineering and adjunct professor in Centre for the environment at IIT Guwahati. Prof. Bora received his PhD from Institute of Genomics and Integrative Biology, Delhi. Prof. Bora teaches Research Methodology, Genome editing and engineering, Advance genetic engineering to undergraduate and postgraduate students of IIT Guwahati. Prof. Bora has an extensive research experience in the areas of Seri-biotechnology, Tissue engineering, genetic engineering and aptamer based molecular diagnostics.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.

The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).

Date and Time of Exams: 29 October 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.

Registration url: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.

Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.

Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Guwahati .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team