Metabolic Engineering

By Prof. Pinaki Sar, Prof. Amit Ghosh   |   IIT Kharagpur, IIT Kharagpur
Learners enrolled: 726
Metabolic engineering is an emerging field of biotechnology/bioprocess engineering which aims towards purposeful modification of cellular (metabolic, gene regulatory, and signalling) processes/networks to achieve desirable goals such as enhanced production of metabolites including pharmaceuticals, biofuels and biochemicals and other biotechnology products.
This course aims to provide fundamental and advanced knowledge in the development of microbial strain for bio production through metabolic engineering.as

B Tech (Biotechnology/Bioengineering); B Sc. (Microbiology, Biotechnology, Botany); M Sc. (Biotechnology / Life Sciences)
PREREQUISITES : Basics of Microbiology, Biochemistry, Genetics
Course Status : Completed
Course Type : Elective
Duration : 8 weeks
Category :
  • Biological Sciences & Bioengineering
  • Bioprocesses
Credit Points : 2
Level : Undergraduate
Start Date : 21 Feb 2022
End Date : 15 Apr 2022
Enrollment Ends : 21 Feb 2022
Exam Date : 24 Apr 2022 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1:Introduction to Metabolic Engineering, Basic concepts; Scopes and Applications; Metabolism overview _1 (Cellular Transport processes, Fueling Reactions)
Week 2:Cellular Metabolism Overview_2 (Biosynthetic reactions, Polymerization, Growth Energetics); Regulation of Metabolic Pathways
Week 3:  Reconstruction of Genome-scale metabolic network
Week 4:Examples of pathway manipulations by metabolic engineering : Ethanol, Aminoacids, antibiotics, vitamines, biopolymers, etc.
Week 5:Examples of pathway manipulations by metabolic engineering : Improvements of cellular properties, Biodegradation,
Week 6:Metabolic Flux Analysis: Flux Balance Analysis (FBA), Flux Variability Analysis, Flux Map
Week 7: Experimental determination of Metabolic Fluxes: Isotope labeled substrate, Isotope mapping Mapping Matrix, Isotope Distribution Vector
Week 8:Application of metabolic Flux Analysis

Books and references

1. Metabolic Engineering, Principles and Methodologies; G N Stephanopoulos, A A Aristidou, J Nielsen
2. Advances in Biochemical Engineering/Biotechnology; Metabolic Engineering, Volume Editor: Jens Nielsen
3. Systems Metabolic Engineering, Methods and Protocols; H S Alper
4. Metabolic Pathway design, A Practical Guide; P Carbonell

Instructor bio

Prof. Pinaki Sar

IIT Kharagpur
Pinaki Sar, Professor, Department of Biotechnology IIT Kharagpur has been engaged in teaching and research on Microbiology and related biotechnology aspects for the past 18 years. His major area of interest lies in the exploration of microbial communities and their catabolic repertoire; and application in environmental sustainability. He uses genomics and metagenomics approaches to understand community/species function. He has been engaged in teaching the subject Metabolic Engineering for over 10 years to the undergraduate (B Tech. Biotechnology and Biochemical Engineering) students of IIT Kharagpur.

Prof. Amit Ghosh

IIT Kharagpur
Dr. Amit Ghosh received his Ph.D. from IISc Bangalore and thereafter did his postdoctoral research for seven years in Metabolic Engineering from Lawrence Berkeley National Laboratory (LBNL) and University of Illinois at Urbana Champaign (UIUC), USA. He has been engaged in research on metabolic engineering, systems and synthetic biology for the last 12 years. He got involved in teaching subjects: Metabolic Engineering, Metabolic Systems Biology to UG & PG students of IIT Kharagpur for the last 4 years.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 24 April 2022 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


Average assignment score = 25% of average of best 6 assignments out of the total 8 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Kharagpur .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

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