Week 1: Essential Quantum Mechanics: Uncertainity Principle, Probabilistic nature of measurement, postulates of qmech, Stern Gerlach equivalent in light, Photon picture (PMT response), Linear Vector Space.
Week 2: Time dependent perturbation theory, Fermi Golden Rule, Transition probability in light matter interaction, Beer Lambert relation, Einestin’s phenomenolgical treatment, A and B coefficients, Spontaneous emission, Origins of fluorescence
Week 3: Nature of Fluorescence, Emisson spectrum, Absorption spectrum, Anisotropy, Life time, FRET
Week 4: Second quantaisation, creation and anhilation operators, Fock states, light matter interaction in Feynman digrams
Week 5: Spontaneous emission orgin, Stimulated Emission origin dependence through Fock states
Week 6: Laser emission, two state, three state and four state laser systems
Week 7: Real world lasers, Charecteristics of laser emission, thershold behavior, Laser gain equation, CW operation, Pulsed lasers, Qswitching, mode locking, Saturable absorber
Week 8: Laser induced fluorescence, optical components (lenses, mirrors, gratings, prisms) and their working principles, Interference filters, dichroic filters, efficiency calculations for SNR improvement, aligning an optical equipment.
Week 9: Intro to optical hardware, common opto-mechanical assemblies, setting up a simple laser based spectrometer using gratings in lab, calibration and acqusition of fluorescein spectra.
Week 10: Principles of photo detection, QE, Dynamic range shot noise, photodetectors – PMTs, photodiodes, photo resistors, understanding common metrics and specs. Detection electronics – preamps, A2Ds
Week 11: Area detectors, CCDs, emCCDs, sCMOS, comparison, read noise, speed and other sensor charecteristics. Theory of Image formations – widefiled microscopy, bright field, phase contrast, DIC and fluorescence microscopy
Week 12: Scanning system: Principles of scanning system, Gaussian light progation and focussing, optical resolution, deinition in xy and z. Measurement and charecetrisation in lab. Scanning as time averaged focus, optical hinges, imaging of hinges, Confocal microscope