Experimental Biotechnology

By Prof. Vishal Trivedi | IIT Guwahati

Learners enrolled: 3007

In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. By the end of this course, student will be able to understand:
1. Basics of Good Lab practices.
2. Understanding different analytical techniques and their applications.
3. Specific Scientific questions and their solutions.
4. Designing new experiments.

INTENDED AUDIENCE : PG/PhD

PREREQUISITES : General Biology 10+2

INDUSTRIES SUPPORT : Biocon https://www.biocon.com/
Jubilant Life Sciences www.jubl.com/
Shantha Biotechnics Ltd
Panacea Biotec
Other companies related to biotechnology

Summary

Course Status :	Completed
Course Type :	Elective
Language for course content :	English
Duration :	12 weeks
Category :	Biological Sciences & Bioengineering Bioprocesses Biosciences
Credit Points :	3
Level :	Postgraduate
Start Date :	26 Jul 2021
End Date :	15 Oct 2021
Enrollment Ends :	09 Aug 2021
Exam Date :	24 Oct 2021 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Week 1: Basics of Laboratory Research:Good Lab Practices, Concept of buffering and Preparation of Solutions and Reagents

Week 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applications

Week 3: Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresis

Week 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC.

Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC.

Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatography

Week 7: Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation.

Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation.

Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques.

Week 10: Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications.

Week 11: Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applications

Week 12: Summary and Conclusions

Books and references

1. H-P Schmauder, M Schweizer and L M Schweizer., Methods in Biotechnology, (eds), Taylor & Francis Publishers, 2002.

2. K. Wilson & J. Walker, Practical Biochemistry: Principles and Techniques. (eds) Cambridge University Press, New York, 1995.

3. Douglas A. Skoog and James J. Leary, Principles of Instrumental Analysis. 4th Edition. Saunders College Publishing, 1992.

4. C. R. Kothari, Research methodology: Methods and Techniques, 3rd Edn., New age International 2014.

5. R. Kumar, Research methodology a step-by-step guide for beginners, Sage Publications, London, 2011.

6. C.G. Thomas, Research methodology and scientific writing, Ane books, Delhi, 2015.

Instructor bio

Prof. Vishal Trivedi

IIT Guwahati

Prof. Trivedi did his Ph.D. from Central Drug Research Institute, Lucknow in the field of Structural Biology. From his postdoctoral research at the Department of Molecular and Cellular Biology, Harvard University and Molecular Oncology Research Institute, Tufts University, Boston, USA, he gained extensive research experience in the field of cell biology, intracellular signal transduction, and immunology. Currently, his laboratory at Department of Biosciences and Bioengineering has an active group working and exploring questions related to malaria parasite biochemistry, the role of novel proteins, development of anti-malarial agents, and lastly understanding factors playing a crucial role in immunomodulation and host pathology in different organs

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
Date and Time of Exams: 24 October 2021 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 75% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Guwahati .It will be e-verifiable at nptel.ac.in/noc.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- NPTEL team

SWAYAM Helpline / Support