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Plant Cell Bioprocessing

By Dr. Smita Srivastava   |   IIT Madras
Learners enrolled: 960
This is a course designed primarily for students in the undergraduate or master’s programs interested in bioprocess development for production of high value products from plant cells and tissue cultures. This course is expected to introduce the student to identify the industrial applications of Plant Cell/Tissue Culture Technology. The student will be able to develop plant cell/tissue culture based bioprocesses for large scale in vitro production of high value phytochemicals. Strategies that can be utilized to improve yield and productivity of phytochemicals from plant cell/tissue cultures with case studies will be discussed. 

INTENDED AUDIENCE : Researchers and students in the area of Plant Biotechnology, Biochemical/Bioprocess Engineering and Biotechnology.
PREREQUISITES : Basic knowledge of Fermentation Technology, Basics in plant cell and tissue culture, plant biotechnology
INDUSTRY SUPPORT : Himalaya Pvt. Ltd., Reliance life sciences,  Dabur
Summary
Course Status : Completed
Course Type : Elective
Duration : 8 weeks
Start Date : 26 Aug 2019
End Date : 18 Oct 2019
Exam Date : 17 Nov 2019 IST
Category :
  • Biological Sciences & Bioengineering
Credit Points : 2
Level : Undergraduate

Page Visits



Course layout

Week 1 Introduction to plant cells 

Week 2:  In-vitro forms of plant tissue cultures for commercial applications and Culture initiation 

Week 3 Somatic embryogenesis and culture preservation ; Secondary metabolism in plant cells: Its role and commercial applications

Week 4  Secondary metabolism in plant cells; Strategies to enhance yield and productivity of plant secondary metabolites in in vitro cell/tissue cultures

Week 5 Strategies to enhance yield and productivity of plant secondary metabolites in in vitro cell/tissue cultures; Biotransformation and Immobilization of plant cell cultures

Week 6 Genetic transformations in plant cells

Week 7 Scale-up considerations in plant cell/tissue cultures

Week 8 Case studies on in vitro production of high-value plant secondary metabolites for commercial applications: A Combinatorial/Integrated approach for synergistic effect on production rates.

Books and references

1.Karl-Hermann Neumann (2009) Plant cell/tissue culture-A tool in Biotechnology: Basics and Application. Springer-Verlag Berlin.
ISBN:978-3-540-93883-5
2.S. Dutta Gupta (2008) Plant Tissue Culture Engineering. Springer.
ISBN:978-1-4020-3594-4
3.J.J. Zhong (2001) Plant cells. Springer. ISBN: 978-3-540-41849-8
4.Plant Cell, Tissue and Organ Culture. Eds., O.L. Gamborg, G.C. Phillips. Springer-VerlagBerlin.
5.Plant Tissue Culture: Theory and Practice. Eds., Bhojawani S.S. and Razdan M.K., Panima Publishing Corporation, New Delhi.
6.Plant Biotechnology, Ed. K.G. Ramawat, S. Chand and Company Ltd., New Delhi.
7.Medicinal Plant Biotechnology, Ed. Ciddi Veeresham, CBC Publishers and Distributors, NewDelhi
8.Chapter 14, Bioprocess considerations in using Plant Cell Cultures. In (eds. Shuler ML& Kargi F) Bioprocess Engineering, Basic concepts. pp-431-435
9.Chapter 5, Cell Cultivation: Plant Cell Cultivations. In (ed. Lee JM) Biochemical Engineering. pp: 118-123.

Instructor bio



Dr. Smita Srivastava has experience in the application of Chemical and Biochemical Engineering principles to facilitate the development of sustainable bioprocesses for commercial production of high-value low-volume phytochemicals. As faculty at IIT Madras, Dr. Smita and her research group have set up a plant cell cultivation facility up to reactor level in the Department of Biotechnology at IIT Madras, to facilitate research on mass cultivation of plant cells/tissues for in vitro production of high-value phytochemicals. Her research experience in the area of bioprocessing for phytochemicals is demonstrated by19 peer reviewed international journal publications, 4 book chapters, 4 Indian patent applications and 27 different international/national conference presentations (as first/corresponding author) in the area of bioprocessing for phytochemicals.To promote research in specialized field like “Plant Cell Bioprocess”, she has formulated and offers a course (as a professional elective) on “Plant Cell Bioprocessing” to undergraduates and postgraduate students at IIT Madras since 2012. She has also been affiliated to the Teaching and Learning Center at IIT Madras (as a core and executive member), since 2011. She has rendered her services as coordinator and resource person in various workshops conducted by TLC for faculty development/training programs and teaching assistant training programmes

Course certificate

  • The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
  • The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
  • Date and Time of Exams: 17 November 2019, Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
  • Registration url: Announcements will be made when the registration form is open for registrations.
  • The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
  • Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE
  • Average assignment score = 25% of average of best 6 assignments out of the total 8 assignments given in the course. 
  • Exam score = 75% of the proctored certification exam score out of 100
  • Final score = Average assignment score + Exam score

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75
  • If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.
  • Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Madras. It will be e-verifiable at nptel.ac.in/noc.
  • Only the e-certificate will be made available. Hard copies are being discontinued from July 2019 semester and will not be dispatched


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