Courses » Cell Culture Technologies

Cell Culture Technologies

About the course

The course will be a short primer to understand how ‘animal cell culture technologies’ have strengthen the bio-medical research from basic research to the modern drug discovery. Animal cell culture was first performed in the very first decade of 19th century. Since then, tremendous development has taken place in this field. The lectures will help the researcher to appreciate the developments during last hundred years and will help them to independently set up cell culture laboratories. For non-biologist, it will be an informal way to demystify the intriguing routes of biomedical research where cell culture is a very ‘potent tool’.

Intended Audience
UG and PG students pursuing biology, biotechnology, zoology and bio-engineering.

Biology at standard 10th (Secondary school examination)

Industries that will recognize this course
Biomedical industries, Biotechnology industry, Drug -discovery industry

4227 students have enrolled already!!

Course Instructor 

Prof.Mainak Das is a faculty of IIT Kanpur India in the department of biological sciences & bioengineering since April 26 2010. He did his bachelors degree (1989-1994) in agriculture from College of Agriculture Indore. Thereafter he did his post graduate degree (1994-1997) in animal physiology from National Dairy Research Institute Karnal India. Following his post graduate studies, he worked as researcher in IISc Bangalore India (1997-1999), University of Neuchatel, Switzerland (1999-2000), University of Clemson, USA (2000-2004) and in University of Central Florida, USA (2004-2010). He did his doctoral studies from College of Medicine of University of Central Florida (2004-2008), while working as a full time employee of the university. He introduced the regular physiology course for the PG students in IIT Kanpur in 2011. He has wide interest in physiology, sensors, energy and bioelectronics and maintains an active research team at IT Kanpur, India. Prof. Das has been working on cell culture technologies, serum free medium development and defined cell culture systems for last 20 years. He has expertise in long term culturing of excitable cells. His doctoral thesis is a complex problem of modern cell culture technology, titled:‘Tissue Engineering The Motoneuron To Muscle Segment Of The Stretch Reflex Arc Circuit Utilizing Micro-fabrication,Interface Design And Defined Medium Formulation’.

You can visit his website by clicking the following link:

Course layout 

Week 1: 
Introduction & biology of cultured cells

Week 2: 
Equipments, aseptic techniques, safety protocols

Week 3: 
Culture vessels & media development

Week 4: 
Serum-free medium development & sterilization

Week 5: 
Primary culture, secondary culture, cloning & selection

Week 6: 
Cell separation, characterization, differentiation & transformation

Week 7: 
Contamination, cryo-preservation & cyto-toxicity

Week 8: 
Organo-typic culture & specialized cell culture techniques

Suggested Reading 

  • Culture of Animal Cells by R Ian Freshney
  • Cell culture technology: Recent advances and future prospects (Euroscicon Meeting Reports Book 1) by Bruserud, Øystein and Astrid Englezou
  • Vertebrate Cell Culture II and Enzyme Technology: Volume 39 (Advances in Biochemical Engineering/ Biotechnology) by A.F. Bückmann and G. Carrea
  • Animal Cell Culture and Technology (The Basics) (Garland Science)) by Michael Butler
  • The Immortal Life of Henrietta Lacks by Rebecca Skloot

  • The exam is optional for a fee.
  • Date of Exam: April 27th 2019 (Saturday).
  • Time of Exam: Morning session 9am to 2 noon; Afternoon session: 2pm to 5pm
  • Registration url: Announcements will be made when the registration form is open for registrations.
  • The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published.

  • Final score will be calculated as : 25% assignment score + 75% final exam score
  • 25% assignment score is calculated as 25% of average of  Best 6 out of 8 assignments
  • E-Certificate will be given to those who register and write the exam and score greater than or equal to 40% final score. Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Kanpur. It will be e-verifiable at nptel.ac.in/noc.