Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.
This course of interactomics focuses on two powerful platforms: 1. Protein microarrays and 2. Label free biosensors involving Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI).
While the former is a high-throughput tool for discovery-based interactomics, the later is proving itself to be an instrumental resource for validation based studies. This course details the overlapping domains of proteomics and interactomics, which form the focus of researchers making an impact in the clinical biomarker and pharmaceutical arena across the globe. Students would benefit hugely from this unique course-model designed for budding scientists as well as seasoned researchers new to this area of research
INTENDED AUDIENCE: Elective course, All UG and PG students can attend this course.It would be applied to B.Sc., M.Sc. and Ph.D.
PREREQUISITES: B.Sc. Or M.Sc.The target audiences of this course are required to have a basic biology and biochemistry background.l
INDUSTRY SUPPORT: GE Healthcare, Pall Corporation, BioRad, Molecular Devices, Promega and other companies dealing with interactomics based.
ABOUT THE INSTRUCTOR:
Dr. Sanjeeva Srivastava is the Group Leader of Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings. Dr. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013]. These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay. Dr. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society to advance the proteomics knowledge for the benefits of global health.
Week 1 :
Lecture 1 : Introduction to Interactomics Lecture 2 : An overview of label-free technologies Lecture 3 : An overview of surface plasmon resonance (SPR) Lecture 4 : An overview of surface plasmon resonance imaging (SPRi) Lecture 5 : Basics of SPR: Surface chemistry
Lecture 11 : Protein-small molecule interaction study: Kinetic analysis Lecture 12 : SPR: Interactive Session-I Lecture 13 : SPR: Interactive Session-II Lecture 14 : An overview of ellipsometry and interferometry techniques Lecture 15 : An introduction to BioLayer Interferometry (BLI) and its applications in protein research Week 4 :
Lecture 16 : Kinetic analysis of protein-protein interaction using BLI Lecture 17 : Label-free quantification of proteins using BLI Lecture 18 : Diffraction-based biosensors I Lecture 19 : Diffraction-based biosensors II Lecture 20 : Nanotechniques in proteomics I Lecture 21 : Nanotechniques in proteomics II Week 5 :
Lecture 22 : High throughputplatforms of interactomics: Protein arrays Lecture 23 : Conventional label based detection techniques for Protein microarrays Lecture 24 : Novel detection techniques for Protein microarrays Lecture 25 : Recombinational cloning and its application for Protein microarrays Lecture 26 : An introduction to Cell-free protein synthesis Week 6 :
Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays Lecture 29 : Digging deeper into NAPPA: Basic Workflow Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery Week 7 :
Lecture 32 : Application of cell free expression protein microarrays in immunological studies Lecture 33 : Basics of microarray image scanning Lecture 34 : Software for Image scanning and data processing Lecture 35 : Microarray Data Analysis: Part I Lecture 36 : Microarray Data Analysis: Part II Week 8 :
Lecture 37 : Application of protein microarray in biomarker discovery-I Lecture 38 : Application of protein microarray in biomarker discovery-II Lecture 39 : Systems biology and networks Lecture 40 : Challenges in proteomics
CERTIFICATION EXAM :
The exam is optional for a fee.
Date and Time of Exams: April 28 (Saturday) and April 29 (Sunday) : Morning session 9am to 12 noon;
Exam for this course will be available in one session on both 28 and 29 April.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published.
Final score will be calculated as : 25% assignment score + 75% final exam score
25% assignment score is calculated as 25% of average of Best 6 out of 8 assignments
E-Certificate will be given to those who register and write the exam and score greater than or equal to 40% final score. Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IITM. It will be e-verifiable at nptel.ac.in/noc.