Courses » Interactomics: Label-free Biosensors & Protein Arrays

Interactomics: Label-free Biosensors & Protein Arrays

About the Course :

Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.

This course of interactomics focuses on two powerful platforms:
1. Protein microarrays and
2. Label free biosensors involving Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI).

While the former is a high-throughput tool for discovery-based interactomics, the later is proving itself to be an instrumental resource for validation based studies. This course details the overlapping domains of proteomics and interactomics, which form the focus of researchers making an impact in the clinical biomarker and pharmaceutical arena across the globe. Students would benefit hugely from this unique course-model designed for budding scientists as well as seasoned researchers new to this area of research

Intended Audience :
Elective course, All UG and PG students can attend this course.It would be applied to B.Sc., M.Sc. and Ph.D.

Pre-requisites :
B.Sc. Or M.Sc.The target audiences of this course are required to have a basic biology and biochemistry background.

Industries that will recognize this course :
GE Healthcare, Pall Corporation, BioRad, Molecular Devices, Promega and other companies dealing with interactomics based 

About the Instructor :

Dr. Sanjeeva Srivastava is the Group Leader of Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings. Dr. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013]. These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay. Dr. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society to advance the proteomics knowledge for the benefits of global health.

Course Plan :
Week 1:
Lecture 1 : Introduction to interactomics
Lecture 2 : An overview of label-free technologies
Lecture 3 : An overview of surface plasmon resonance (SPR)
Lecture 4 : An overview of surface plasmon resonance imaging
Lecture 5 : Basics of SPR: Surface chemistry

Week 2:

Lecture 6 : Basics of SPR: Experimental design
Lecture 7 : Protein immobilization for protein-protein interaction studies
Lecture 8 : Protein-protein interaction study: Binding analysis
Lecture 9 : Protein-protein interaction study: Kinetic analysis
Lecture 10 : Protein-small molecule interaction study: Immobilization & binding analysis

Week 3:

Lecture 11 :  Protein-small molecule interaction study: Kinetic analysis
Lecture 12 :  SPR: Interactive Session-I
Lecture 13 :  SPR: Interactive Session-II
Lecture 14 :  An overview of ellipsometry and interferometry techniques
Lecture 15 :  An introduction to BioLayer Interferometry (BLI) and its applications in protein research

Week 4:

Lecture 16 :  Kinetic analysis of protein-protein interaction using BLI
Lecture 17 :  Label-free quantification of proteins using BLI
Lecture 18 :  Diffraction-based biosensors I
Lecture 19 :  Diffraction-based biosensors II
Lecture 20 :  Nanotechniques in proteomics I
Lecture 21 :  Nanotechniques in proteomics II

Week 5:

Lecture 22 : High throughputplatforms of interactomics: Protein arrays
Lecture 23 : Conventional label based detection techniques for Protein microarrays
Lecture 24 : Novel detection techniques for Protein microarrays
Lecture 25 : Recombinational cloning and its application for Protein microarrays
Lecture 26 : An introduction to Cell-free protein synthesis

Week 6:
Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA
Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
Lecture 29 : Digging deeper into NAPPA: Basic Workflow
Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery

Week 7:

Lecture 32 : Application of cell free expression protein microarrays in immunological studies
Lecture 33 : Basics of microarray image scanning
Lecture 34 : Software for Image scanning and data processing
Lecture 35 : Microarray Data Analysis: Part I
Lecture 36 : Microarray Data Analysis: Part II

Week 8:

Lecture 37 : Application of protein microarray in biomarker discovery-I
Lecture 38 : Application of protein microarray in biomarker discovery-II
Lecture 39 : Systems biology and networks
Lecture 40 : Challenges in proteomics

Certification Exam :
  • The exam is optional for a fee.Exams will be on 23 April 2017.
  • Time : Shift 1: 9 AM -12 Noon;Shift 2 : 2 PM-5 PM
  • Any one shift can be chosen to write the exam for a course.
  • Registration URL : Announcements will be made when the registration form is open for registrations.
  • The online Registration form has to be filled and the certification exam fee needs to be paid.More details will be made available when the exam registration form is published.
Certificate :
  • Final score will be calculated as : 25% assignment score + 75% final exam score.
  • 25% assignment score is calculated as 25% of average of 8 weeks course : Best 6 out of 8 assignments
  • E-Certificate will be given to those who register and write the exam and score greater than or equal to 40% final score. Certificate will have your name, photograph and the score in the final exam with the breakup. It will have the logos of NPTEL and IIT Bombay. It will be e-verifiable at nptel.ac.in/noc.