Courses » Downstream Processing

Downstream Processing


The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing.
The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied field
This course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.


Basics of biochemical engineering and thermodynamics


Prof. Mukesh Doble at the Department of Biotechnology at IIT Madras. Has previously worked in Imperial chemical Industries (ICI) and General Electric (GE) for 20 years. Areas of research are Biomaterials, Biopolymers, and Drug design. Published 270 papers and 10 books and filed 10 patents (including two US). Has delivered on line video courses in Downstream processes and Biostatistics



Week 1

 Lec-01 Introduction

 Lec-02 Mass balance, Heat Balance, flow sheet

 Lec-03 Costing

Week 2

Lec-04 Costing (continued), Physical and chemical principles in Downs stream

Lec-05 Problems in Mass balance, flow sheet

Lec-06 Cell Breakage

Week 3

Lec-07 Cell Breakage (continued)

 Lec-08 Solid Liquid Separation

 Lec-09 Solid Liquid Separation (continued)

Week 4

Lec-10 Solid Liquid separation-problems

Lec-11 Pre-treatment and Filters

 Lec-12 Adsorption

Week 5

Lec-13 Adsorption (continued)

 Lec-14 Adsorption (continued)

 Lec15 Adsorption (continued)

Week 6

 Lec-16 Liquid-Liquid Extraction

Lec-17 Liquid-Liquid extraction (continued)

 Lec-18 Liquid-Liquid extraction (continued)

Week 7

Lec-19 Liquid-Liquid extraction (continued)

Lec-20 Reversed micellar and aqueous two phase extraction

 Lec-21 Membranes

Week 8

Lec-22 Membranes (continued)

Lec-23 Membranes (continued)

Lec-24 Membranes (continued)

Week 9

 Lec-25 Precipitation

 Lec-26 Chromatography

 Lec-27 Chromatography (continued)

Week 10

Lec-28 Chromatography (continued)

Lec-29 Chromatography (continued)

 Lec-30 Chromatography (continued)

Week 11

Lec-31 Chromatography (continued)

 Lec-32 Chromatography (continued)

 Lec-33 Crystallisation

Week 12

Lec-34 Drying

 Lec-35 Drying and Distillation

Lec-36  Future trends, Summary of the course


Name of the course:  Downstream Processing
Course duration : 12 weeks (In weeks)
Dates of exams : 23 April 2017
  • Time of exam : Shift 1: 9am-12 noon; Shift 2: 2pm-5pm
  • Any one shift can be chosen to write the exam for a course.
Final List of exam cities will be available in exam registration form.
Exam registration url - Will be announced shortly


1.Belter, P.A. and Cussler, E.L. Hu, W.S (1988), Bioseparation: Downstream processing for Biotechnology, Wiley, New York.
2.Ladisch, M.R., (2001), Bioseparation Engineering: Principles, Practice and Economics, Wiley, Interscience.

  • The exam is optional for a fee. Exams will be on 23 April 2017 
  • Time: Shift 1: 9am-12 noon; Shift 2: 2pm-5pm
  • Any one shift can be chosen to write the exam for a course.
  • Registration url: Announcements will be made when the registration form is open for registrations.
  • The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. 

  • Final score will be calculated as : 25% assignment score + 75% final exam score
  • 25% assignment score is calculated as 25% of average of Best 8 out of 12 assignments
  • E-Certificate will be given to those who register and write the exam and score greater than or equal to 40% final score. Certificate will have your name, photograph and the score in the final exam with the breakup. It will have the logos of NPTEL and Institute which is coordinating. It will be e-verifiable at nptel.ac.in/noc.