Prof. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings.
Prof. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013].
These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay.
Prof. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society advance the proteomics knowledge for the benefits of global health.
The exam is optional. Exams will be on 24 April 2016 and 30 April 2016.
Registration URL: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee of Rs 1000, needs to be paid.
Certificate will be given to those who register and write the exam. Certificate will have your name, photograph and the score in the final exam.
It will also have the logos of NPTEL and IIT Bombay. It will also be e-verifiable on the nptel.ac.in/noc website.
L1. Introduction to amino acids
L2. Introduction to proteins
L3. Protein folding & misfolding
L4. Protein purification techniques
L5. Introduction to proteomics
Lab session-1.1 Demonstration of protein-protein interaction using label-free biosensors
Week-2: Gel-based proteomics and Sample Preparation
L6. Systems biology and proteomics
L7. Sample preparation and pre-analytical factors
L8. Sample preparation: Pre-analytical factors (contd.)
L9. Sample preparation: Protein extraction and quantification
L10. One-dimensional electrophoresis
Lab session-2.1 Sample preparation: Tissue sample preservation technology
Lab session-2.2 Protein/peptide pre-fractionation using OFF-GEL FRACTIONATOR
Week-3: Two-dimensional gel electrophoresis (2-DE)
L11. 2-DE: Rehydration, IEF & Equilibration
L12. 2-DE: Second dimension, staining & destaining
L13. 2-DE: Gel analysis
L14. 2-DE: Applications
L15. 2-DE: Applications (contd.) & Challenges
Lab session-3.1 Demonstration of gel analysis using IQTL software
Week-4: Difference in gel electrophoresis (DIGE) & Mass Spectrometry
L16. 2D-DIGE: Basics
L17. 2D-DIGE: Data analysis
L18. 2D-DIGE: Applications
L19. Protein identification using MALDI-TOF/TOF
L20. Proteomics experiment data analysis & challenges
Lab session-4.1 Demonstration of Q-TOF MS technology