Courses » Proteins and Gel-Based Proteomics

Proteins and Gel-Based Proteomics


This course introduces to the basic biology of proteins and gel-based proteomics. To obtain better understanding of cellular processes & regulation, there has been an increasing interest in studying proteomics. Proteomics techniques aim to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. These proteomic techniques typically aim to elucidate the expression, interaction, and cellular function of proteins.

The course will provide the basic understanding of amino acids and proteins. It would also provide detailed information on gel-based proteomic techniques such as SDS-PAGE and 2-DE. Some of the limitation of 2-DE has been overcome by advanced Difference gel electrophoresis (DIGE) technique, which will be discussed in this course. Protein identification using MALDI-TOF/TOF mass spectrometry will also be discussed. 2-DE in combination with mass spectrometry has become very powerful tool for proteomics applications and students can learn these tools by attending this course. 


Core course
All UG and PG students can attend this course.
It would be applied to B.Sc., M.Sc. and Ph.D


B.Sc. Or M.Sc.
The target audiences of this course are required to have a basic biology and biochemistry background.

404 students have enrolled already!!


Prof. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings.

Prof. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013].

These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay.

Prof. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society advance the proteomics knowledge for the benefits of global health.

Click here to view Faculty Profile: http://www.bio.iitb.ac.in/~sanjeeva/

About the Instructor: https://youtu.be/sb4faypvWwk


The exam is optional. Exams will be on 24 April 2016 and 30 April 2016.
Time: 2pm-5pm

Tentative list of exam cities: http://nptel.ac.in/noc/pdf/Exam%20City-list.pdf

Registration URL: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee of Rs 1000, needs to be paid.


Certificate will be given to those who register and write the exam. Certificate will have your name, photograph and the score in the final exam. 

It will also have the logos of NPTEL and 
IIT Bombay. It will also be e-verifiable on the nptel.ac.in/noc website.


Week-1: Basics of amino acids and proteins

L1. Introduction to amino acids

L2. Introduction to proteins

L3. Protein folding & misfolding

L4. Protein purification techniques

L5. Introduction to proteomics

Lab session-1.1 Demonstration of protein-protein interaction using label-free biosensors

Week-2: Gel-based proteomics and Sample Preparation

L6. Systems biology and proteomics

L7. Sample preparation and pre-analytical factors

L8. Sample preparation: Pre-analytical factors (contd.)

L9. Sample preparation: Protein extraction and quantification

L10. One-dimensional electrophoresis

Lab session-2.1 Sample preparation: Tissue sample preservation technology

Lab session-2.2 Protein/peptide pre-fractionation using OFF-GEL FRACTIONATOR

Week-3: Two-dimensional gel electrophoresis (2-DE)

L11. 2-DE: Rehydration, IEF & Equilibration

L12. 2-DE: Second dimension, staining & destaining

L13. 2-DE: Gel analysis

L14. 2-DE: Applications

L15. 2-DE: Applications (contd.)Challenges

Lab session-3.1 Demonstration of gel analysis using IQTL software

Week-4: Difference in gel electrophoresis (DIGE) & Mass Spectrometry

L16. 2D-DIGE: Basics

L17. 2D-DIGE: Data analysis

L18. 2D-DIGE: Applications

L19. Protein identification using MALDI-TOF/TOF

L20. Proteomics experiment data analysis & challenges

Lab session-4.1 Demonstration of Q-TOF MS technology