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Courses » Mass spectrometry based proteomics

Mass spectrometry based proteomics

ABOUT THE COURSE

Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio. MALDI and ESI- coupled with mass analyzers are commonly used mass spectrometer configuration in proteomics. The human proteome draft was decoded by using high-resolution liquid chromatography coupled with mass spectrometry.  

In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.

INTENDED AUDIENCE

Core course
All UG and PG students can attend this course.
It would be applied to B.Sc., M.Sc. and Ph.D

PRE-REQUISITES

B.Sc. Or M.Sc.
The target audiences of this course are required to have a basic biology and biochemistry background.

569 students have enrolled already!!

COURSE INSTRUCTOR


Prof. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings.

 Prof. Srivastava has considerable experience for teaching proteomics courses and conducting hands-on crash-courses on proteomics at IIT Bombay and many other institutes. Apart from the regular research work, Dr. Srivastava has been actively involved in the development of various proteomics e-Learning & Open-Learning curriculums and Virtual Lab initiatives at IIT Bombay generated with an intention to disseminate high-quality educational materials on proteomics at a global scale [Ray et al., PLoS Biol. 2012; Ray et al., J Proteomics. 2012; Srivastava et al., Nature 2013].

These Virtual Lab initiatives have been included as a part (Tutorial- IPTP 14) of the International Proteomics Tutorial Programme (supported by Human Proteome Organization (HUPO) and European Proteomics Association (EuPA)). Dr. Srivastava’s teaching efforts has provided him Excellence in Teaching Award in IIT Bombay.

 Prof. Srivastava envisions designing novel-framework by linking proteomics and big data for the disruptive innovation by collective intelligence and actionable foresight. He believes strongly in trans-generational capacity building in science, bioengineering and 21st century knowledge society advance the proteomics knowledge for the benefits of global health.

Click here to view Faculty Profile: Prof. Sanjeeva Srivastava
Click here to view Faculty Introduction: https://www.youtube.com/watch?v=sb4faypvWwk&feature=youtu.be

CERTIFICATION EXAM

The exam is optional.

Exams will be on 1 November 2015 and 8 November, 2015. 

Time: 1pm-4pm

The list of cities where the exam will be conducted will be available in the registration form.

Registration URL: Announcements will be made when the registration form is open for registrations.

The online registration form has to be filled and the certification exam fee of Rs 1000, needs to be paid.

CERTIFICATE

Certificate will be given to those who register and write the exam. Certificate will have your name, photograph and the score in the final exam. 

It will also have the logos of NPTEL and IIT Bombay. It will also be e-verifiable on the 
nptel.ac.in/noc website.



SYLLABUS OUTLINE

Week 1: Proteomics introduction and sample preparation


Lec 1: Introduction to proteomics
Lec 2: Proteomics and sample preparation
Lec 3: Bacterial protein extraction
Lec 4: In-gel digestion
Lec 5: Fundamentals of mass spectrometry

Week 2: Basics of mass spectrometry

Lec 6: Chromatography technologies
Lec 7: Liquid chromatography
Lec 8: Mass spectrometry: Ionization sources
Lec 9: Mass spectrometry: Mass analyzers
Lec 10: MALDI sample preparation and analysis

Week 3: Quantitative proteomics

Lec 11: Introduction to quantitative proteomics
Lec 12: Hybrid mass spectrometry configurations
Lec 13: SILAC: In vivo labeling
Lec 14: iTRAQ: In vitro labeling
Lec 15: TMT: In vitro labeling


Week 4: Proteomics and systems biology

Lec 16: Quantitative proteomics data analysis
Lec 17: Proteomics and systems biology-I
Lec 18: Proteomics and systems biology-II
Lec 19: Proteomics applications
Lec 20: Advances and challenges in proteomics